Journal: Journal of Stem Cell Research & Therapy

Article Title: Mesenchymal Stem Cells Suppress Muscle Atrophy Induced by Hindlimb Suspension

doi: 10.4172/2157-7633.1000266

Figure Lengend Snippet: Figure 3: The effect of MSCs on muscle mass and lactate levels in rats with HS-induced muscle atrophy. (A) Soleus muscle weight/body weight (mg/g) at one and two weeks (*P<0.05, compared to HS+Saline group; #P<0.05, compared to HR+Saline group). (B) Lactate levels in the soleus muscle at 1 week after MSCs injection (&P<0.05, compared to control group; *P<0.05, compared to the HS+Saline group; #P<0.05, compared to HR+Saline group).

Article Snippet: Human adipose-derived mesenchymal stem cells (AD-MSCs; PCS500-011, passage #6) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in Dulbecco’s modified Eagle medium (DMEM; Hyclone laboratories, Inc., Logan, UT, USA) supplemented with 10% FBS, 100 mg/mL streptomycin, and 100 IU/mL penicillin.

Techniques: Saline, Injection, Control

Journal: Journal of Stem Cell Research & Therapy

Article Title: Mesenchymal Stem Cells Suppress Muscle Atrophy Induced by Hindlimb Suspension

doi: 10.4172/2157-7633.1000266

Figure Lengend Snippet: Figure 4: Histological evaluation of MSCs engrafted in soleus muscle. (A) MSCs injection ameliorates the histological phenotype. Muscle sections were stained with hematoxylin and eosin (H&E). (B) The cross-sectional area (CSA) of soleus muscle fibers. Differences in muscle fiber size were visually confirmed by H&E staining of the CSA at 1 and 2 weeks (*P<0.05, compared to HS+Saline group; #P<0.05, compared to HR+Saline group). (Original magnification 200X, scale bars=50 μm).

Article Snippet: Human adipose-derived mesenchymal stem cells (AD-MSCs; PCS500-011, passage #6) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in Dulbecco’s modified Eagle medium (DMEM; Hyclone laboratories, Inc., Logan, UT, USA) supplemented with 10% FBS, 100 mg/mL streptomycin, and 100 IU/mL penicillin.

Techniques: Injection, Staining, Saline

Journal: Journal of Stem Cell Research & Therapy

Article Title: Mesenchymal Stem Cells Suppress Muscle Atrophy Induced by Hindlimb Suspension

doi: 10.4172/2157-7633.1000266

Figure Lengend Snippet: Figure 5: The effect of MSCs on expression of muscle-specific markers in HS-induced atrophic muscle. The mRNA expression of skeletal muscle actin (A) and desmin (B) were confirmed in soleus muscles (*P<0.05, compared to HS+Saline group; #P< 0.05, compared to HR+Saline group).

Article Snippet: Human adipose-derived mesenchymal stem cells (AD-MSCs; PCS500-011, passage #6) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in Dulbecco’s modified Eagle medium (DMEM; Hyclone laboratories, Inc., Logan, UT, USA) supplemented with 10% FBS, 100 mg/mL streptomycin, and 100 IU/mL penicillin.

Techniques: Expressing, Muscles, Saline

Journal: Journal of Stem Cell Research & Therapy

Article Title: Mesenchymal Stem Cells Suppress Muscle Atrophy Induced by Hindlimb Suspension

doi: 10.4172/2157-7633.1000266

Figure Lengend Snippet: Figure 6: Improvement of muscle regeneration in muscle atrophy after MSCs injection and mechanism analysis. (A) Immunofluorescence analysis using paraffin sections of soleus muscles from the Control, HS+Saline, HS+UC and HR+UC group rats at 2 weeks. Desmin is labeled with Cy3 (red), UC-MSCs are labeled with alexa 488 (green), and nuclei are stained with DAPI (blue). (Original magnification 200X, scale bars=50 μm). (B-G) Immunoblot analysis of the expression of PI3K/AKT signaling pathway. Representative Western blots showing the expression of whole protein level and phosphorylated (p) forms of AKT and FoxO-1/3a, and the expression of total form of MAFbx (Atrogin-1) and MuRF-1 in the soleus muscle. The expression of phosphorylated AKT (C), FoxO-1/3a (D and E), MAFbx (F) and MuRF-1 (G) normalized to β-actin expression (&P<0.05, compared to control group; *P<0.05, compared to HS+Saline group). Error bars represent standard deviation.

Article Snippet: Human adipose-derived mesenchymal stem cells (AD-MSCs; PCS500-011, passage #6) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in Dulbecco’s modified Eagle medium (DMEM; Hyclone laboratories, Inc., Logan, UT, USA) supplemented with 10% FBS, 100 mg/mL streptomycin, and 100 IU/mL penicillin.

Techniques: Injection, Immunofluorescence, Muscles, Control, Saline, Labeling, Staining, Western Blot, Expressing, Standard Deviation